Liquid Chromatography-Triple Quadrupole Mass Spectrometer (LC-MS/MS)

• API-ESI (Atmospheric Pressure Ionization - Electrospray Ionization) Techniques
• PDA Detector range: 200-800nm
• Ultra-Fast Mass Spectrometry (UFMS) for rapid analysis, quick polarity switching and stable signal detection
• Mass Range : m/z 2 to 2,000
• Resolution : R < 0.7 u FWHM and adjustable to 0.5 u
• Scan speed : Max. 30,000 u/sec (in all scan modes) (0.1 u step: 300,000 data points/sec)
• Positive-negative ion polarity switching time : 5 msec
• MRM transition speed : Max. 555 channels/sec
• Mass Stability : 0.05 Da/24 hr
• Quantification at ppb level in MRM method

LC-MS/MS is used in various fields such as

• Pharmaceutical Industry - Drug testing, Contamination analysis
• Food Industry - Food Quality Assessment.
• Environmental Application – Soil and Water Pollution Analysis
• LC-MS/MS is also used in metabolomics, proteomics, and genomics studies, as well as in the analysis of natural products and biofuels.
• LCMS is applied in many industries such as pharmaceuticals, biopharmaceuticals, forensic, industrial, food and environmental sector
• For clinical research, the analysis of drugs, vitamins and minerals in whole blood, plasma, serum and urine

PRP 417
Pearl Research Park
Centre for Nanobiotechnology

Dr. John Thomas
Associate Professor
Centre for Nanobiotechnology
VIT, Vellore-632014
Email: cnbt.lcmsms@vit.ac.in
Phone: 0416 220 4214