REAL-TIME PCR

Medical and Diagnostic Applications
Infection Diagnosis: RT-PCR is a primary tool for detecting viral infections, such as COVID-19, influenza, HIV, and Hepatitis C, by identifying the viral RNA in patient samples. Cancer Monitoring: It helps track cancer progression and treatment response by measuring changes in gene expression levels in tumors. Genetic Disorder Screening: The technique can detect specific genetic mutations associated with certain inherited conditions, such as Lesch-Nyhan syndrome.

Biological Research Applications
Gene Expression Profiling: RT-PCR allows for the precise quantification of gene expression levels to study gene function and cellular processes. RNA Interference (RNAi) Validation: Researchers use it to confirm that target genes have been effectively "knocked down" in RNAi experiments. Cloning and DNA Manipulation: The process generates complementary DNA (cDNA) from RNA, which can then be cloned and used in further molecular biology studies.

Food Safety and Environmental Monitoring
Pathogen Detection: In food and environmental testing, RT-PCR rapidly detects bacterial and viral pathogens like Salmonella, Listeria, and E. coli in food, water, and soil samples. GMO Detection: It is used to identify the presence of genetically modified organisms (GMOs) in food products. Ingredient Authentication: RT-PCR can also confirm the presence or absence of specific ingredients, such as pork in halal or kosher foods.

Dr. M.A. Jayasri
Associate Professor Senior
School of Bio Sciences and Technology,
Mob: +91 9944024206
Email : jayasri.ma@vit.ac.in