Fluorescence Spectroscopy

Home Facilities Fluorescence Spectroscopy

Make: Agilent

Model: Cary Eclipse

  1. Excitation:
    A light source, often ultraviolet light, is directed at the sample.
  2. Excitation State:
    Molecules in the sample absorb photons of light and enter a higher energy "excited state".
  3. Emission:
    As the excited molecules return to their ground state, they release the absorbed energy by emitting a photon of light. This emitted light is the fluorescence.
  4. Detection:
    A fluorescence detector (a type of spectrofluorometer) measures the intensity and wavelengths of the emitted light.
  5. Spectra:
    The data collected can be plotted as:
    • Fluorescence Emission Spectrum: Shows the intensity of emitted light at different wavelengths for a fixed excitation wavelength.
    • Fluorescence Excitation Spectrum: Shows fluorescence intensity at a specific emission wavelength across a range of excitation wavelengths.
    • Excitation-Emission Matrix (EEM): A 2D plot of fluorescence intensity as a function of both excitation and emission wavelengths.
  • Pharmaceuticals: Quality control and analysis of drugs.
  • Biochemistry: Studying biological molecules and their environments.
  • Environmental Science: Analyzing soil constituents and organic pollutants.
  • Art Conservation: Non-invasively characterizing materials in artworks.
  • Materials Science: Investigating optoelectronic materials and semiconductor devices.
Room No.: 113
SMV ( Hexagon Building)
School of Bio Sciences and Technology
Dr. Podili Koteswaraiah
School of Bio Sciences and Technology,
Vellore Institute of Technology - Vellore Campus,
Thiruvalam Road, Vellore, Tamil Nadu, India.
Landline: 0416-220 2556
Mobile: +91- 9840934792
Faculty In-charge E-mail ID:koteswaraiahpodili@vit.ac.in
S. No. Experiment Details UG /PG Students from Academic Institutions (in INR) Research Scholar from Academic Institution (in INR) Project Staff from Academic Institution (in INR) Faculty Members from Academic Institutions (in INR) Research Scientist from R&D Organizations (in INR) Industry Personnel from Industry (in INR)
1 Per Sample 500 500 500 500 500 500
* Charges for per sample in INR (+ applicable GST)