Make: Agilent
Model: Cary Eclipse
- Excitation:
A light source, often ultraviolet light, is directed at the sample.
- Excitation State:
Molecules in the sample absorb photons of light and enter a higher energy "excited state".
- Emission:
As the excited molecules return to their ground state, they release the absorbed energy by emitting a photon of light. This emitted light is the fluorescence.
- Detection:
A fluorescence detector (a type of spectrofluorometer) measures the intensity and wavelengths of the emitted light.
- Spectra:
The data collected can be plotted as:
- Fluorescence Emission Spectrum: Shows the intensity of emitted light at different wavelengths for a fixed excitation wavelength.
- Fluorescence Excitation Spectrum: Shows fluorescence intensity at a specific emission wavelength across a range of excitation wavelengths.
- Excitation-Emission Matrix (EEM): A 2D plot of fluorescence intensity as a function of both excitation and emission wavelengths.
- Pharmaceuticals: Quality control and analysis of drugs.
- Biochemistry: Studying biological molecules and their environments.
- Environmental Science: Analyzing soil constituents and organic pollutants.
- Art Conservation: Non-invasively characterizing materials in artworks.
- Materials Science: Investigating optoelectronic materials and semiconductor devices.
Room No.: 113
SMV ( Hexagon Building)
School of Bio Sciences and Technology
S. No.
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Experiment Details
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UG /PG Students from Academic Institutions (in INR)
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Research Scholar from Academic Institution (in INR)
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Project Staff from Academic Institution (in INR)
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Faculty Members from Academic Institutions (in INR)
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Research Scientist from R&D Organizations (in INR)
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Industry Personnel from Industry (in INR)
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1
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Per Sample
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500
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500
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500
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500
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500
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500
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* Charges for per sample in INR (+ applicable GST)