Fast Protein Liquid Chromatography

Fast Protein Liquid Chromatography, a laboratory technique used to separate and purify proteins, peptides, and other biomolecules. It utilizes a stationary phase of resin-filled columns and a mobile liquid buffer to achieve high-resolution separation based on various properties, such as size, charge, or affinity. FPLC operates at medium pressure and employs automated systems for buffer control, sample injection, and fraction collection, enabling efficient and reproducible purification of biomolecules.

• Proteins & Peptides: FPLC is widely used to purify specific proteins and peptides from complex mixtures.
• Nucleic Acids: It can also be used for the rapid purification of RNA, synthetic oligonucleotides, and plasmid DNA.
Clinical Applications
• Disease Diagnosis: FPLC can separate proteins in bodily fluids like cerebrospinal fluid (CSF) and urine to identify disease markers.
• Protein Profiling: It is used to analyze protein profiles for conditions such as tubular proteinuria and to detect changes in CSF protein composition.
• Diagnostic Markers: FPLC helps in the diagnosis of diseases like beta-thalassemia by separating specific blood proteins, according to Unacademy.
Research & Biotechnology
• Drug Discovery: The technique is employed in drug development processes to separate and analyze biomolecules.
• Enzymology: FPLC is a valuable tool for isolating, purifying, and characterizing enzymes.
• Recombinant Protein Production: It is used to purify recombinant proteins and antibodies for various uses.
Other Applications
• Food Industry: FPLC is used to analyze the nitrogenous constituents in beer, which are relevant to its foam stability.
• Pest Control: The technique has been used to purify animal venoms for various applications.