High Performance Liquid Chromatography

• Flow rate range: typically, 0.001 to 10 mL/min (depends on pump configuration)
• Operating pressure: up to 600 bar (≈ 8700 psi)
• High precision quaternary/binary gradient pumping system
• Wide dynamic range UV detector for accurate quantification
• Refractive Index Detector (RID) for compounds lacking UV absorbance
• Excellent baseline stability and noise reduction
• Compatible with aqueous and organic solvent systems
• Fast data acquisition with advanced system control

Detection System

UV Detector

• Variable wavelength UV detection (typically 190–600 nm)
• Suitable for chromophore-containing compounds
• High sensitivity and reproducibility

Refractive Index Detector (RID)

• Universal detector for non-UV active compounds
• Ideal for:
• Sugars
• Alcohols
• Polymers
• Requires isocratic elution (not suitable for gradient)

Software

• Controlled by Agilent OpenLab CDS

Features:

• Automated data acquisition and processing
• Peak integration and quantification
• Method development and validation
• System suitability testing

• Separation and purification of organic compounds
• Pharmaceutical analysis
• Food and sugar analysis (RID-based)
• Environmental sample analysis

Room No.: G03
CO2 Research and Green Technologies Centre (CO2RGTC),
Vellore Institute of Technology (VIT), Vellore.

DR. G. VELVIZHI
CO2 Research and Green Technologies Centre (CO2RGTC),
G03, CO2RCTC,
VELLORE INSTITUTE OF TECHNOLOGY - VELLORE CAMPUS,
THIRUVALAM ROAD, VELLORE – 632 014, TAMIL NADU, INDIA.
Office Phone: +91 – 416 – 220 2503/2504
Faculty in charge email id: velvizhi.g@vit.ac.in