School of Bio Sciences & Technology

• For basic flowcytometric analysis like cell cycle, Apotosis with Annexin-V antibody etc., Quick laser uptime enables relatively faster start-up.
• With Cytexpert software support for preliminary analysis.
• Three lasers and adequate filters to analyse samples of excitation in 16 colours. Multi-colour analysis possible.

Cell cycle analysis, Apoptosis, Immunophenotyping etc.

Room No.: 130A
Hexagon (SMV) building
School of Bio Sciences and Technology

Dr. K. M. Gothandam
Professor Grade 2
School of Bio Sciences and Technology
VIT Vellore, TN-632014.
Email: sbst.flowcyto@vit.ac.in

• FTIR is a Fourier Transform Infra-Red, that can detect a range of functional groups and is sensitive to changes in molecular structure.
• FTIR provide information on the basis of chemical composition and physical state of the whole sample

Identifying the chemical molecules , interaction of proteins, metabolites with DNA and RNA.

Room No.: 113
Hexagon (SMV) Building
School of Bio Sciences and Technology

Dr. R. Siva
Professor Grade 2
School of Bio Sciences and Technology
VIT Vellore, TN-632014.
Email: sbst.ftir@vit.ac.in

Animal organ samples and researchers working on experimental animals.

Room No.: 07A
CBMR Building
School of Bio Sciences and Technology

Dr. V. Pragasam,
Professor Grade 2 & Dean
School of Bio Sciences and Technology
VIT Vellore, TN-632014.
Email: sbst.histopathology@vit.ac.in

• Preparative system capable of eluting 120ml/min
• Equipped with programmed fraction collector
• Analytical system equipped with auto sampler having 110 sample slots
• PDA detector 200-800 nm
• RI detector for analysing non chromophore compounds

• Large scale separation and purification of biomolecules.
• Quantitative estimation of metabolites with low concentration.

Room No.: 204
Hexagon(SMV) building
School of Bio Sciences and Technology

Dr. C. Rajasekaran,
Professor Grade 1
School of Bio Sciences and Technology
VIT Vellore, TN-632014.
Email: sbst.hplc@vit.ac.in

• One of the most flexible, reliable, and cost-efficient separation technique ideally suited for the analysis of botanicals and herbal drugs.
• High sample throughput since several samples can be chromatographed simultaneously can be coupled with LC-MS.

An ideal screening tool for detection of adulterations, evaluation and monitoring of cultivation, harvesting, and extraction processes and testing of stability. Used in analysing the purity and efficacy of pharmaceutical preparations and dosage forms. It is a globally accepted practical solution to characterize small molecules in quality assessment. It is used for purity control of chemicals, pesticides, steroids, and water analysis. It is also widely used for analysis of vitamins, water-soluble food dyes, pesticides in fruits, vegetables, and other food stuffs.

Ground floor- CDMM building
School of Bio Sciences and Technology

Dr. AbulKalam Azad Mandal
Higher Academic Grade,
School of Bio Sciences & Technology,
VIT Vellore, TN-632014.
Email: sbst.hptlc@vit.ac.in

• Measure storage modulus of semisolid materials and soft solids.
• Measure viscosity of viscous fluids, flow curves of fine powders.

Rheological study of materials.

Bioinspired Design Lab
Room No.: G29A
Hexagon(SMV) Building
School of Bio Sciences and Technology

Dr. Debasish Mishra,
Associate Professor(Sr.)
School of Bio Sciences and Technology
VIT Vellore, TN-632014.
Email: sbst.rheometer@vit.ac.in

• PCR, Gene expression analysis, precision melt curve analysis with all available channels.
• Calibrated for High Resolution Melting (HRM) curve analysis.
• Data analysis in single setup.
• Data export options available.

Gene expression analysis

Room No.: 244
Hexagon(SMV) building
School of Bio Sciences and Technology

Dr. K. M. Gothandam,
Professor Grade 2,
School of Bio Sciences and Technology
VIT Vellore, TN-632014.
Email: sbst.rtpcr@vit.ac.in

• ? 3, 00,000x Magnification
• Lab 6 Cathode filament
• Variable pressure mode, Back scattered secondary Electron, X Ray Spectroscopy
• Ultrastructure of surfaces:
  

  Organism, metals, materials, powdered samples, polymers, nanomaterial, textile, fibres, soil, rubber/plastics, concrete samples, welded metals, food sample, etc.,

• Chemical elemental composition by EDX
• All elements of periodic tables
• Qualitative
• Quantitative analysis

Materials and Biological samples.

Room No.: 130A
SMV ( Hexagon Building)
School of Bio Sciences and Technology

Dr. AbulKalam Azad Mandal
Higher Academic Grade
School of Bio Sciences & Technology
VIT Vellore, TN-632014.
Email: sbst.sem@vit.ac.in

Efficient separations from samples as small as 175ml up to 32.4ml and at speeds of up to 150,000 rpm and more than 1,000,000xg

Sub cellular fractionation from the crude extract, affinity purification of membrane vesicles, separation of DNA components, colloid separation and virus purification.

Room No.: 113
SMV Building
School of Bio Sciences and Technology

Dr. V. Pragasam,
Professor Grade 2 & Dean
School of Bio Sciences & Technology
VIT Vellore, TN-632014.
Email: sbst.ultracentri@vit.ac.in

The HPLC-PDA-MS helps in,

• Obtaining the mass of the analyte
• Absorption spectrum of the analyte
• Evaluating the purity of the analyte
• Purification of a specific analyte
• Quantification of the analyte against a known standard
• Comparative profiling of the metabolite composition
• Authenticating the existence of a specific metabolite.

School of Bio Sciences and Technology,
SMV 124 A,
VELLORE INSTITUTE OF TECHNOLOGY - VELLORE CAMPUS,
THIRUVALAM ROAD, VELLORE, TAMIL NADU, INDIA.,/p>

Dr. G. Jayaraman
School of Bio Sciences and Technology,
SMV 124 A,
VELLORE INSTITUTE OF TECHNOLOGY - VELLORE CAMPUS,
THIRUVALAM ROAD, VELLORE, TAMIL NADU, INDIA.

Landline: 0416-220 2683
Mobile: +91- 9944796904

Faculty In-charge E-mail ID: gjayaraman@vit.ac.in
Facility E-mail ID: sbst.pdams@vit.ac.in

• Forensic Science: Detecting latent bloodstains at crime scenes.
• Medical Diagnostics: High-sensitivity detection and quantification of antigens and biomarkers in clinical tests.
• Environmental Monitoring: Measuring the concentrations of pollutants like nitrogen oxides in the atmosphere.
• Biological Imaging: Visualizing cellular processes or detecting specific DNA or protein sequences.

• Pulse waveform control; impedance correction; reproducible current delivery; suitable for bacteria, yeast, fungi, mammalian cells.

• Gene delivery; DNA/RNA transfection; molecular biology and cell biology research.

No.: 113, SMV (Hexagon Building),
School of Bio Sciences and Technology,
VIT, Vellore

1. Sterilization in situ (SIP) Able to sterilize the fermenter vessel, piping for inputs like air/gas, stirrer shaft etc., without disassembly. This ensures aseptic conditions. Scigenics lists "lab scale in situ sterilizable stirred tank fermenters" in its product range.
2. Controlled temperature Heating and cooling systems (jacketed or coil) to maintain culture temperature precisely. Likely with +/- tolerances (±0.1-0.5°C) for sensitive cultures.
3. Agitation / mixing A stirrer (top‑driven shaft, possibly bottom‑driven or magnetically sealed depending on model) to maintain homogeneity, ensure oxygen transfer, suspend cells if needed.
4. Automated control & software A controller (could be PLC or dedicated bioreactor controller) for setting setpoints, alarms, logging
5. Cleanability and aseptic design Features for CIP (Cleaning In Place) and sterilization: smooth surfaces, minimal “dead volumes”, properly sealed seals, ports for cleaning, possibly splash plates, etc.
6. Safety features Pressure relief or venting, safety interlocks, explosion/vacuum protection if the design calls for it (depending on anaerobic/aerobic process), temperature overshoot protection.
7. Flexibility of culture mode Ability to run batch and fed‑batch fermentation

• Digital rotational viscometer for precise viscosity measurements
• Wide viscosity range: 15–2,000,000 cP (depending on spindle and speed)
• Speed range: 0.1 to 200 rpm with multiple selectable speeds
• Touchscreen interface with programmable test modes
• Temperature monitoring with optional probe for thermo-sensitive samples
• Data logging and USB connectivity for easy transfer and reporting

The Ametek LVDV2T Viscometer is used across pharmaceuticals, food, cosmetics, polymers, and research labs to measure and control fluid viscosity for stability, consistency, and quality assurance.

• Polymer Solutions
• Pharmaceuticals
• Food & Beverages
• Paints & Coatings
• Chemical & Petrochemicals
• Research & Development

• Advanced UV-Vis spectrophotometer with multicell capability
• Wavelength range: typically 190–1100 nm (covers UV and visible spectrum)
• Simultaneous measurement of multiple samples using a multicell holder
• Temperature-controlled measurements for kinetic and thermal stability studies
• High photometric accuracy, repeatability, and linear dynamic range
• Fast scanning and data acquisition suitable for both routine and research applications

• Nucleic Acid & Protein Quantification
• Enzyme Kinetics
• Material Characterization
• Quality Control
• Spectral Analysis
• Thermal Studies

• Advanced UV-Vis spectrophotometer with multicell capability
• Wavelength range: typically 190‑1100 nm (covers UV and visible spectrum)
• Simultaneous measurement of multiple samples using a multicell holder
• Temperature-controlled measurements for kinetic and thermal stability studies
• High photometric accuracy, repeatability, and linear dynamic range
• Fast scanning and data acquisition suitable for both routine and research applications

• Quantification of nucleic acids and proteins ‑ Measuring DNA, RNA, and protein concentrations and purity.
• Enzyme kinetics & biochemical assays ‑ Monitoring reaction rates and substrate/product formation.
• Molecular characterization ‑ Studying absorbance spectra of biomolecules, chromophores, and cofactors.
• Quality control in pharma, food, and environment ‑ Analysis of drugs, additives, pollutants, and dyes.
• Nanomaterial and chemical analysis ‑ Optical characterization of nanoparticles, polymers, and chemical compounds.

• Provides a controlled environment: It maintains optimal conditions for the growth of microorganisms.
• Allows for large-scale production: Fermenters are designed to grow microorganisms and produce metabolites in large quantities.
• Enables controlled chemical processes: It facilitates biochemical processes by providing nutrients and removing waste products.

• Food and Beverage:
  • Production of fermented foods like yogurt, cheese, and soy products.
  • Production of beverages such as beer and wine.
• Pharmaceuticals:
  • Production of essential medications, including antibiotics, insulin, and vaccines.
  • Production of therapeutic proteins, enzymes, and vitamin B12.
• Chemicals:
  • Production of industrial chemicals like ethanol, butanol, and acetone.
  • Production of organic acids, such as citric acid.
• Biotechnology:
  • Used to grow and culture microorganisms or cells for various biotechnological applications.
  • Creation of value-added products through the use of genetically engineered microorganisms.
• Waste Treatment:
  • Used to decompose organic materials and convert them into more useful products.

Medical and Diagnostic Applications
Infection Diagnosis: RT-PCR is a primary tool for detecting viral infections, such as COVID-19, influenza, HIV, and Hepatitis C, by identifying the viral RNA in patient samples. Cancer Monitoring: It helps track cancer progression and treatment response by measuring changes in gene expression levels in tumors. Genetic Disorder Screening: The technique can detect specific genetic mutations associated with certain inherited conditions, such as Lesch-Nyhan syndrome.

Biological Research Applications
Gene Expression Profiling: RT-PCR allows for the precise quantification of gene expression levels to study gene function and cellular processes. RNA Interference (RNAi) Validation: Researchers use it to confirm that target genes have been effectively "knocked down" in RNAi experiments. Cloning and DNA Manipulation: The process generates complementary DNA (cDNA) from RNA, which can then be cloned and used in further molecular biology studies.

Food Safety and Environmental Monitoring
Pathogen Detection: In food and environmental testing, RT-PCR rapidly detects bacterial and viral pathogens like Salmonella, Listeria, and E. coli in food, water, and soil samples. GMO Detection: It is used to identify the presence of genetically modified organisms (GMOs) in food products. Ingredient Authentication: RT-PCR can also confirm the presence or absence of specific ingredients, such as pork in halal or kosher foods.

1. Excitation:
   A light source, often ultraviolet light, is directed at the sample.
2. Excitation State:
   Molecules in the sample absorb photons of light and enter a higher energy "excited state".
3. Emission:
   As the excited molecules return to their ground state, they release the absorbed energy by emitting a photon of light. This emitted light is the fluorescence.
4. Detection:
   A fluorescence detector (a type of spectrofluorometer) measures the intensity and wavelengths of the emitted light.
5. Spectra:
   The data collected can be plotted as:
   • Fluorescence Emission Spectrum: Shows the intensity of emitted light at different wavelengths for a fixed excitation wavelength.
   • Fluorescence Excitation Spectrum: Shows fluorescence intensity at a specific emission wavelength across a range of excitation wavelengths.
   • Excitation-Emission Matrix (EEM): A 2D plot of fluorescence intensity as a function of both excitation and emission wavelengths.

• Pharmaceuticals: Quality control and analysis of drugs.
• Biochemistry: Studying biological molecules and their environments.
• Environmental Science: Analyzing soil constituents and organic pollutants.
• Art Conservation: Non-invasively characterizing materials in artworks.
• Materials Science: Investigating optoelectronic materials and semiconductor devices.

• Microvolume UV-Vis spectrophotometer (1‑2 µL sample requirement)
• DNA/RNA quantification at 260 nm with purity ratios (A260/A280, A260/A230)
• Protein quantification at 280 nm or 205 nm
• Broad concentration range with auto path-length adjustment
• Surface tension-based pedestal measurement (no cuvettes needed)
• Rapid results within seconds, high sensitivity and accuracy

• Compact benchtop freeze dryer for routine use
• Ice condenser capacity: 2.5 kg
• Ice condenser temperature: ‑55 °C
• User-friendly control interface
• Suitable for aqueous samples and small-volume drying

• Routine laboratory freeze-drying of biological/aqueous samples
• Training and introduction to lyophilization techniques
• Small-scale drying tasks for research and teaching labs

Fast Protein Liquid Chromatography, a laboratory technique used to separate and purify proteins, peptides, and other biomolecules. It utilizes a stationary phase of resin-filled columns and a mobile liquid buffer to achieve high-resolution separation based on various properties, such as size, charge, or affinity. FPLC operates at medium pressure and employs automated systems for buffer control, sample injection, and fraction collection, enabling efficient and reproducible purification of biomolecules.

• Proteins & Peptides: FPLC is widely used to purify specific proteins and peptides from complex mixtures.
• Nucleic Acids: It can also be used for the rapid purification of RNA, synthetic oligonucleotides, and plasmid DNA.
Clinical Applications
• Disease Diagnosis: FPLC can separate proteins in bodily fluids like cerebrospinal fluid (CSF) and urine to identify disease markers.
• Protein Profiling: It is used to analyze protein profiles for conditions such as tubular proteinuria and to detect changes in CSF protein composition.
• Diagnostic Markers: FPLC helps in the diagnosis of diseases like beta-thalassemia by separating specific blood proteins, according to Unacademy.
Research & Biotechnology
• Drug Discovery: The technique is employed in drug development processes to separate and analyze biomolecules.
• Enzymology: FPLC is a valuable tool for isolating, purifying, and characterizing enzymes.
• Recombinant Protein Production: It is used to purify recombinant proteins and antibodies for various uses.
Other Applications
• Food Industry: FPLC is used to analyze the nitrogenous constituents in beer, which are relevant to its foam stability.
• Pest Control: The technique has been used to purify animal venoms for various applications.